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EN9F10
EN9F10
規(guī)格:
貨期:
編號:B176737
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EN9F10
商品貨號 B176737
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma: B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain BALB/c
Applications
The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate.
The antibody can be used to determine the toxic potential of agricultural commodities and forensic samples involving most ergot alkaloids.
Storage Conditions liquid nitrogen vapor phase
Derivation
The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate.
The antibody detects ergopeptine and lysergic acid derivatives.
Genes Expressed
immunoglobulin; monoclonal antibody; against ergonovine (an ergot alkaloid)
Cellular Products
immunoglobulin; monoclonal antibody; against ergonovine (an ergot alkaloid)
Comments
The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate.
The antibody detects ergopeptine and lysergic acid derivatives.
The antibody can be used to determine the toxic potential of agricultural commodities and forensic samples involving most ergot alkaloids.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at  2  to 5  x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.  Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype mouse IgG2b
Name of Depositor RA Shelby
Deposited As mouse (B cell); mouse (myeloma)
References

Shelby RA, Kelley VC. Detection of ergot alkaloids in tall fescue by competitive immunoassay with a monoclonal antibody. Food Agric. Immunol. 3: 169-177, 1991.

Shelby RA, Kelley VC. Detection of ergot alkaloids from Clavicops species in agricultural products by competitive ELISA using a monoclonal antibody. J. Agric. Food Chem. 40: 1090-1092, 1992.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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