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pSNATCH

規格:

價格:詢價

編號:B176918

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

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產品名稱

pSNATCH

商品貨號

B176918

Designations

pSNATCH

Depositors

C Hovens

培養基

Biosafety Level

Vector Information

Size (kb): 5.0819997787475590

Vector: pSNATCH (phagemid)

Promoters: Promoter CMV

Construction: pSCTEV, pSuperCATCH-NLS

Marker(s):ampR

Construct size (kb): 5.081999778747559

Features: marker(s): ampR
other: VP16 activation domain
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: SfuI...XbaI
signal peptide coding region: SV40 NLS
terminator: SV40
epitope tag: FLAG

Applications

encodes removable tag for protein isolation

expression vector

vector for assessing nuclear localization signals

vector permitting construction of fusion proteins

vector permitting production of single-stranded DNA

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.1; HindIII--5.1; PstI--3.7, 1.4.

The vector was constructed as follows: a 120bp VP16 fragment from pSCTEV Gal4p/VP16 was ligated to adaptors and cloned into BamHI cut pSuperCATCH-NLS.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Buchert M, et al. Useful vectors for the two-hybrid system in mammalian cells. BioTechniques 23: 396-402, 1997. PubMed: 9298205