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asb4/134
asb4/134
規格:
價格:詢價
編號:B176978
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
asb4/134
商品貨號
B176978
Designations
asb4/134
GenBank Number

X15617

培養基
Species
Mus musculus, mouse
Depositors
AJ Ouellette
Applications
This probe has been used to isolate several cryptdin-related cDNAs from an intestinal cDNA library.
Vector
Construct size (kb): 4.679999828338623
Insert
DNA: cDNA
Insert lengths(kb): 0.3149999976158142
Tissue: adult small intestine
Gene product: defensin-related cryptdin peptide [Defcr]
Alleles: b, a, c
Insert Size (kb)
0.315
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following sites separated by (bp): (PstI)- 46- AvaII- 54- HinfI- 12- BglII- 2- Sau3A, XhoII- 15- Sau3A- 45- PstI- 18- RsaI- 54- RsaI- 19- BanI- 48- (PstI).
Restriction digests of the clone give the following sizes (kb): PstI--4.5, 0.16 (doublet); EcoRI--4.8; HindIII--4.8; BamHI--4.8; AvaI--4.8; PvuI/NdeI--2.9, 1.8.
The cryptdin mRNA (450 - 500 nt in length) is abundant in Paneth cells and can be detected in the testis and the brain.
The gene maps to the proximal region of chromosome 8, within 1.28-21.27 cM of the Xmv-26 locus.
Contains a open reading frame encoding a highly polar protein of 70 aa with all 6 cysteine residues in the COOH-terminal half. There are about 100 untranslated nucleotides 3', including poly(A).
The insert lacks 115 bp of 5'-terminal sequence of the mRNA (which has a total size of 0.45 kb). This probe has been used to isolate several cryptdin-related cDNAs from an intestinal cDNA library.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Ouellette AJ, et al. Localization of the cryptdin locus on mouse chromosome 8. Genomics 5: 233-239, 1989. PubMed: 2571573

Ouellette AJ, et al. Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium. J. Cell Biol. 108: 1687-1695, 1989. PubMed: 2715173

Ouellette AJ, Lualdi JC. A novel mouse gene family coding for cationic, cysteine-rich peptides: regulation in small intestine and cells of myeloid origin. J. Biol. Chem. 265: 9831-9837, 1990. PubMed: 2351676

Andre J Ouellette, personal communication

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