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Poterioochromonas malhamensis (Pringsheim) Peterfi
Poterioochromonas malhamensis (Pringsheim) Peterfi
規格:
貨期:
編號:B177101
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Poterioochromonas malhamensis (Pringsheim) Peterfi
商品貨號 B177101
Deposited As Ochromonas malhamensis Pringsheim
Application
assay of vitamin B12 cobalamin, cobalamins
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format test tube
Type Strain no
Comments
characterization of mitochondrial genome
comparison of methods of vitamin analysis
water-soluble vitamins in cells and spent culture supernatants
photosynthetic
Medium ATCC® Medium 891: Ochromonas medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11532 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, loosen the cap, one half turn and incubate on a 15 degree horizontal slant at 20-25 C under 150 foot candles of light (40-50 (Einsteins/meter2 /second). Cells tend to clump on sides and bottom of tube. Subculture every 7-10 d. Screw the cap on tightly, invert the tube several times and aseptically transfer a 0.1 ml aliquot to a fresh tube of medium. Incubate as above. For Vitamin B12 assay, use B12 Ochromonas medium.
Subcultivation
Protocol: ATCCNO: 11532 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, loosen the cap, one half turn and incubate on a 15 degree horizontal slant at 20-25 C under 150 foot candles of light (40-50 (Einsteins/meter2 /second). Cells tend to clump on sides and bottom of tube. Subculture every 7-10 d. Screw the cap on tightly, invert the tube several times and aseptically transfer a 0.1 ml aliquot to a fresh tube of medium. Incubate as above. For Vitamin B12 assay, use B12 Ochromonas medium.
Name of Depositor SH Hunter
Chain of Custody
ATCC <<--SH Hunter<<--E.G. Pringsheim 1950
References

Coleman AW, et al. Identification of the mitochondrial genome in the chrysophyte alga Ochromonas danica. J. Protozool. 38: 129-135, 1991.

Analytical microbiology. New York: Academic Press; 1963.

Analytical microbiology. New York: Academic Press; 1963.

Voight MN, et al. Comparison of Protozoan and conventional methods of Vitamin analysis. J. Food Sci. 44: 729-734, 1979.

Voigt MN, et al. Vitamin analysis by microbial and protozoan organisms: response to food preservatives and neutralization salts. J. Food Sci. 44: 724, 1979.

Baker ER, et al. Water-soluble vitamins in cells and spent culture supernatants of Poteriochronous stipitata, Euglena gracilis and Tetrahymena thermophila. Arch. Microbiol. 129: 310-313, 1981.

Ghione M, Dell'Orto P. Human polypeptidic hormone-like substances in microorganisms. Microbiologica 6: 315-326, 1983. PubMed: 6319976

Ford JE. The microbiological assay of 'vitamin B12'; the specificity of the requirement of Ochromonas malhamensis for cyanocobalamin. Br. J. Nutr. 7: 299-306, 1953.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479