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當(dāng)前位置: 首頁 > ATCC代理 > Poterioochromonas malhamensis (Pringsheim) Peterfi B177101
Poterioochromonas malhamensis (Pringsheim) Peterfi
Poterioochromonas malhamensis (Pringsheim) Peterfi
規(guī)格:
價格:詢價
編號:B177101
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Poterioochromonas malhamensis (Pringsheim) Peterfi
商品貨號
B177101
Deposited As
Ochromonas malhamensis Pringsheim
Application
assay of vitamin B12 cobalamin, cobalamins
培養(yǎng)基
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
test tube
Type Strain
no
Comments
characterization of mitochondrial genome
comparison of methods of vitamin analysis
water-soluble vitamins in cells and spent culture supernatants
photosynthetic
Medium
ATCC® Medium 891: Ochromonas medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11532 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, loosen the cap, one half turn and incubate on a 15 degree horizontal slant at 20-25 C under 150 foot candles of light (40-50 (Einsteins/meter2 /second). Cells tend to clump on sides and bottom of tube. Subculture every 7-10 d. Screw the cap on tightly, invert the tube several times and aseptically transfer a 0.1 ml aliquot to a fresh tube of medium. Incubate as above. For Vitamin B12 assay, use B12 Ochromonas medium.
Subcultivation
Protocol: ATCCNO: 11532 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, loosen the cap, one half turn and incubate on a 15 degree horizontal slant at 20-25 C under 150 foot candles of light (40-50 (Einsteins/meter2 /second). Cells tend to clump on sides and bottom of tube. Subculture every 7-10 d. Screw the cap on tightly, invert the tube several times and aseptically transfer a 0.1 ml aliquot to a fresh tube of medium. Incubate as above. For Vitamin B12 assay, use B12 Ochromonas medium.
Name of Depositor
SH Hunter
Chain of Custody
ATCC <<--SH Hunter<<--E.G. Pringsheim 1950
References

Coleman AW, et al. Identification of the mitochondrial genome in the chrysophyte alga Ochromonas danica. J. Protozool. 38: 129-135, 1991.

Analytical microbiology. New York: Academic Press; 1963.

Analytical microbiology. New York: Academic Press; 1963.

Voight MN, et al. Comparison of Protozoan and conventional methods of Vitamin analysis. J. Food Sci. 44: 729-734, 1979.

Voigt MN, et al. Vitamin analysis by microbial and protozoan organisms: response to food preservatives and neutralization salts. J. Food Sci. 44: 724, 1979.

Baker ER, et al. Water-soluble vitamins in cells and spent culture supernatants of Poteriochronous stipitata, Euglena gracilis and Tetrahymena thermophila. Arch. Microbiol. 129: 310-313, 1981.

Ghione M, Dell'Orto P. Human polypeptidic hormone-like substances in microorganisms. Microbiologica 6: 315-326, 1983. PubMed: 6319976

Ford JE. The microbiological assay of 'vitamin B12'; the specificity of the requirement of Ochromonas malhamensis for cyanocobalamin. Br. J. Nutr. 7: 299-306, 1953.

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