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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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BM-N
BM-N
規格:
價格:詢價
編號:B177293
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BM-N
商品貨號
B177293
Organism
Bombyx mori, silkworm
Product Format
frozen
培養基
Culture Properties
mixed: adherent and suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
transfection host
Host suitable for infection by Bombyx mori nuclear polyhedrosis virus (BmNPV), and may be used to propagate BmNPV-based expression vectors.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
The line should be cultivated at 27C.
Complete Growth Medium
The base medium for this cell line is IPL-41 Insect Cell Culture Medium with glutamine (available from Sigma or Gibco). To make the complete growth medium, add the following components to the base medium:
  • 2.6 g/L Tryptose Phosphate Broth (TPB)
  • 0.069 mg/L ZnSO4·7H2O
  • 3.86 mg/L AlCl3·6H2O
  • 5 mg/L chlorophenol red
  • 2g/L NaCl
  • 10% heat-inactivated insect grade fetal bovine serum

    • Adjust pH to 6.3 to 6.4 by adding 1N NaOH
    Adjust osmolarity to 317 to 360 by adding extra NaCl

    The IPL-41 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation. (In Vitro 17: 495-509, 1981)
Subculturing
Medium Renewal: Once per week
Gently resuspend attached cells in old culture medium by pipetting old medium across the cells on the floor of the flask.
Cryopreservation
Culture medium, 90%; DMSO, 10%
Culture Conditions
Temperature: 27.0°C
Name of Depositor
Daiichi Seiyaku Co., Ltd.
Deposited As
Bombyx mori
U.S. Patent Number
References

Maeda S, et al. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature 315: 592-594, 1985. PubMed: 2989694

. . J. Seric. Sci. Jpn. 53: 547-548, 1984.

Maeda S, Furusawa M. Method of producing substances in living silkworms. US Patent 5,118,616 dated Jun 2 1992

Maeda S, et al. Method of producing peptides using baculovirus vectors in cultured cells. US Patent 5,110,729 dated May 5 1992

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