| Vector Information |
Size (kb): 5.0780000686645510 DESCRIPTION OF VECTOR:
Intact vector size: 5.078
Type of vector: plasmid
Cloning sites: SacI SacII NotI XbaI SpeI BamHI SmaI EcoRI EcoRV ClaI SalI
XhoI ApaI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pUC, Tn903
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
centromere: CEN6
insert detection: lacZ'
marker(s): kanR, HIS3
promoter: lac
replicon: pMB1, ARSH4 Vector: pJA70 (plasmid) Promoters: Promoter lac Construction: pUC, Tn903 Marker(s):kanR,HIS3 Construct size (kb): 5.078000068664551 Features: insert detection: lacZ'
marker(s): kanR, HIS3
promoter: lac
replicon: pMB1, ARSH4
centromere: CEN6 |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--2.2, 1.8, 1.2; HindIII--3.1, 1.9, 0.2; KpnI--2.9, 2.1; EcoRI--5.1. YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffron's laboratory. Permits visual detection of recombinants by the lacZalpha complementation system. The order of the major features in this plasmid is: lacZ'/MCS - CEN6 - ARS H4 - HIS3 - kanR - pMB1 ori. |
