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Construct size (kb): 6.099999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CYP2A6
Genomic copy number: unique
Gene name: cytochrome P450, subfamily IIA (phenobarbital-inducible)
Contains complete coding sequence?: Y
Chromosome: 19
Tissue: liver
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.727
Cross references: DNA Seq. Acc.: M33318
Nucleotides 1-1726 of the insert correspond to
nucleotides 22-1748 of M33318.

Insert lengths(kb): 1.726999998092651

Tissue: liver

Gene product: cytochrome P450, subfamily IIA (phenobarbital-inducible)(coumarin 7-hydroxylase) [CYP2A6]

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.7, 1.7; BamHI--3.3, 1.7, 0.95; HindIII--6.3; PstI--2.8, 1.7, 1.65; XbaI--6.3.

The open reading frame of this insert is 4 aa shorter than that described in the sequence record M33318, but the first Met encoded here appears to support initiation and the protein product is functional.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.13; PvuII--0.13; ClaI--0.49; StuI--0.7, 1.33; BamHI--0.78; SacI--1.34.

The construct contains two copies of the insert in a head-to-tail orientation.