| 產品名稱 |
MEF (CF-1) IRR |
| 商品貨號 |
B177565 |
| Organism |
Mus musculus, mouse |
| Tissue |
Embryo |
| Cell Type |
Fibroblast |
| Product Format |
frozen |
| Morphology |
Fibroblast |
| Culture Properties |
Adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
14 days gestation embryo |
| Gender |
male and female mixed |
| Strain |
CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms |
| Applications |
Irradiated cells for use as feeder layer |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
male and female mixed |
| Comments |
These cells have been growth-arrested by irradiation with 10,000 rads. The cells will begin to deteriorate 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%
This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
|
| Subculturing |
Medium Renewal: Twice a week or when pH decreases |
| Culture Conditions |
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
ATCC |
| Year of Origin |
2004 |
| References |
Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.
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