Comments
These cells have been growth-arrested by irradiation with 10,000 rads. The cells will begin to deteriorate 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 15%
This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
