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p5G1
p5G1
規格:
價格:詢價
編號:B177677
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p5G1
商品貨號
B177677
Designations
p5G1
Species
Homo sapiens, human
培養基
Depositors
L Tsui
Vector
Construct size (kb): 7.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 3.5
Tissue: adult liver
Gene product: crystallin, gamma polypeptide pseudogene 1 [CRYGP1]
Alleles: B1, A1, A2, C1, C2, B2
Insert Size (kb)
3.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 3.3; BamHI--7.3, <1.0; PstI--4.1, 2.7, <1.0;
EcoRI--5.4, 2.3; purified DNA: HindIII--4.4, 3.3; BamHI--7.3, <1.0; PstI--4.1, 2.7, <1.0.
The insert corresponds to the entire sequence of the gene.
The probe hybridizes to four EcoRI digested human DNA fragments: 1.2 kb and 4.0 kb bands corresponding to the G1psi gene, an 18 kb band containing the G2psi gene, and a 13 kb band corresponding to the G3 and G4 genes.
The insert is a HindIII fragment derived from lambda5G1.
Enzyme(s) not detecting polymorphism: EcoRI, BamHI, HindIII, XbaI.
References

Willard HF, et al. Assignment of human gamma crystallin multigene family to chromosome 2. Somatic Cell Mol. Genet. 11: 511-516, 1985. PubMed: 2994242

Meakin SO, et al. Structural and evolutionary relationships among five members of the human gamma-crystallin gene family. Mol. Cell. Biol. 5: 1408-1414, 1985. PubMed: 4033658

Lubsen NH, et al. A locus for a human hereditary cataract is closely linked to the gamma-crystallin gene family. Proc. Natl. Acad. Sci. USA 84: 489-492, 1987. PubMed: 3025877

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