Contains a synthetic consensus Escherichia coli RNA polymerase promoter, HindIII CAT gene cartridge, and pBR327 amp and tet resistance genes.
Identical to pXJ002 (ATCC 37183) except the transcription start site is altered from CCC to CAT.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Rossi JJ, et al. Biological expression of an Escherichia coli sonsesus sequence promoter and some mutant derivatives. Proc. Natl. Acad. Sci. USA 80: 3203-3207, 1983. PubMed: 6304698
