| 產品名稱 |
UACC-2727 |
| 商品貨號 |
B177787 |
| Organism |
Homo sapiens, human |
| Tissue |
ovary |
| Cell Type |
adherent |
| Product Format |
frozen |
| Morphology |
epithelial-like |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
ovarian cancer |
| Age |
64 |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
Cytogenetic analysis at passage 0 (T92-017) revealed a modal number of 66-70 with a range of 39-113 chromosomes per cell. Numerical alterations include -4, -9, +12, -15, -19, -19, +20, -21, -21, -22, -22, -x. Structural alterations are i(1q) [5], +del(1)(p31) [6], +der(3)t(2;3)(p11;p11) [5], add(5)(q31) [6], del(7)(q112) [6], +i(8q) [5], add(11)(p15) [6], add(13)(p12) [4], i(15q) [4], der(16)t(3;?;16)(q11;?;q24) [6], i(17q) [6], ider(19)(q10)add(19)(q134) [5], add(22)(q13) [5] with 3 unidentified markers. Note the isochromosome of 1q, 8q, 15q, 17q and 19q. Passage 10. |
| Derivation |
The human ovarian cancer cell line UACC-2727, was derived from an omentum mass of an untreated 63-year-old female. Pathology confirmed that the specimen was consistent with metastatic papillary serous carcinoma, stage 4. |
| Antigen Expression |
MAK-6, expressed |
| Receptor Expression |
Estrogen Receptor, not expressed, progesterone receptor (PR), not expressed |
| Oncogene |
HER-2/neu, moderate expression; EGFR, normal expression; n-Myc, expressed |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 5%
- 0.01 mg/ml transferrin (final conc.)
- 0.01 mg/ml insulin (final conc.)
- 5 µg/mL (55 U/ml) catalase (final conc.)
- 3.6 µg/mL (0.01 mM) hydrocortisone (final conc.)
- 70 µg/mL (0.5mM) o-phosphoethanolamine (final conc.)
- 10 ng/ml human recombinant epidermal growth factor (EGF) (final conc.)
- 3 ng/ml (0.01 μM) estradiol (final conc.)
- 0.8 ng/ml (1 pM) Na-L-thyroxine (final conc.)
- extra 2 mM glutamine
Note: Do not filter complete medium.
|
| Subculturing |
These cells are cultured on collagen I coated vessels. Add 5 µg/cm2 collagen I (BD Biosciences, Cat. No.354236 or equivalent) to culture vessels and incubate at room temperature for 1 hour. Remove collagen solution and rinse vessels 3 times with a balanced salt solution. Vessels may be used immediately or air dried and stored at 2-8°C for up to one week under sterile conditions. Alternatively, commercially available pre-coated Collagen I vessels, such as BD BioCoat Cellware (BD Biosciences, Cat. No. 356524 for 75 cm2 flask) or equivalent, may be used.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of solutions for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (DPBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 10 minutes. Discard supernatant.
6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new collagen I coated culture vessels. An inoculum of 1.0 X 104 to 4.0 X 104 viable cells/cm2 is recommended.
7. Incubate cultures at 37.0°C.
|
| Culture Conditions |
Temperature: 37°C
Atmosphere: Atmospheric air |
| STR Profile |
TH01: 7
D5S818: 9, 11
D13S317: 8, 13
D7S820: 10
D16S539: 11
CSF1PO: 10, 11
Amelogenin: X
vWA: 14, 15
TPOX: 11 |
| Name of Depositor |
K Brown |
| Year of Origin |
1992 |
