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pALCA1SIFN

規(guī)格:

價格:詢價

編號:B177797

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

pALCA1SIFN

商品貨號

B177797

Designations

pALCA1SIFN

Species

Homo sapiens, human

培養(yǎng)基

Depositors

Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.

Applications

in another host, produces protein interferon, alpha 2

Vector

Construct size (kb): 5.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pALCA1S
Intact vector size: 4.500
Type of vector: plasmid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: EcoRI, BamHI
Polylinker sites:
Other unique sites: HindIII
Construction: pDG6 (ATCCC 53169)
Host range: Escherichia coli; filamentous fungi
Features (with orientation and position when available):
promoter: alcA, ->
signal peptide coding region: synthetic, ->
MCS: XbaI...EcoRI
marker(s): ampR
replicon: pMB1
marker(s): ARGB
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNA2
Genomic copy number: unique
Gene name: interferon, alpha 2
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 p22
Type of DNA: cDNA
Insert 5' end: BglII
Insert 3' end: EcoRI
Insert size (kb): 0.9
Cross references:

Insert lengths(kb): 0.8999999761581421

Gene product: interferon, alpha 2 [IFNA2]

Target Gene: interferon, alpha 2

Insert Size (kb)

0.900

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--4.5, 0.9; HindIII--5.4, EcoRI--5.4.

This plasmid may be difficult to isolate and digest. If this problem occurs, increase the final concentration of ampicillin in the growth mediun to 1 mg/ml.

Transformants secrete protein, inducible by threonine and repressible by glucose.

References

Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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