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GTpZapBglII
GTpZapBglII
規(guī)格:
價格:
編號:B177798
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
GTpZapBglII
商品貨號
B177798
Designations
GTpZapBglII
GenBank Number

M34353

培養(yǎng)基
Species
Homo sapiens, human
Depositors
MH Wigler
Vector
Construct size (kb): 6.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 3.619999885559082
Tissue: glioblastoma SW-1088 cell line
Gene product: avian UR2 sarcoma virus oncogene (v-ros) homolog [ROS]
Alleles: A1, A2, A1, A2
Insert Size (kb)
3.620
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert includes the following restriction sites (nt positions according to the published sequence): BglII--4382; EcoRI--4623, 6183, 6649, 6845, 6965; HindIII--5438, 6939; BamHI--5631; PstI--5640; BglI--6333; AccI--6376, 6409.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.5, 0.5, 0.3: BamHI--2.9, 2.3, 1.3; HindIII--3.3, 1.5, 1.1, 0.6; PstI--5.2, 1.3; NotI--3.6, 2.9.
Extends from the BglII site at nt 4382 to approximately nt 8000.
The open reading frame extends from nt 207 to nt 7247. There is approximately 1 kb of 3' untranslated sequence which has not been fully characterized.
Subcloned from lambdagt10.165 which was constructed by adding NotI linkers to a blunt-ended BglII fragment.
References

Birchmeier C, et al. Characterization of ROS1 cDNA from a human glioblastoma cell line. Proc. Natl. Acad. Sci. USA 87: 4799-4802, 1990. PubMed: 2352949

Michael H Wigler, personal communication

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