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p627
p627
規格:
貨期:
編號:B177799
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 p627
商品貨號 B177799
Designations p627
GenBank Number

X03484

Species Homo sapiens, human
Depositors UA Rapp
Applications
produces protein murine leukemia viral (v-raf-1) oncogene homolog 1 Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.900000095367432
Tissue: fetal liver
Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1( Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1) [RAF1]
Alleles: B, M, B1, A1, A2, B2
Target Gene: murine leukemia viral (v-raf-1) oncogene homolog 1
Insert Size (kb) 2.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: Frozen
Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--2.9, 2.7; HindIII--5.25, 0.55; BglII--5.7; XbaI--3.5, 2.2.
RAF1 shows linkage to Von Hippel-Lindau disease (VHL) with a LOD score of 4.38 with theta = 0.11.
Haplotype frequencies for the TaqI and BglI polymorphisms suggest only slight linkage disequilibrium between the two sites.
Significant linkage has been demonstrated to von Hippel-Lindau disease (theta = 0.0, z = 2.6).
Insert contains PvuII (2), HindII, SalI (2), StuI (3), SphI, BglII, EcoRV, and Xba sites.
References

Hall JM, et al. Oncogenes and human breast cancer. Am. J. Hum. Genet. 44: 577-584, 1989. PubMed: 2564734

Bonner TI, et al. Structure and biological activity of human homologs of the raf/mil oncogene. Mol. Cell. Biol. 5: 1400-1407, 1985. PubMed: 2993863

Elliott RW, et al. Localization of the rhodopsin gene to the distal half of mouse chromosome 6. Genomics 6: 635-644, 1990. PubMed: 2341153

Vance JM, et al. Confirmation of linkage in von Hippel-Lindau disease. Genomics 6: 565-567, 1990. PubMed: 2328994

Bonner TI, et al. The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene. Nucleic Acids Res. 14: 1009-1015, 1986. PubMed: 3003687

Seizinger BR, et al. Von-Hippel Lindau disease maps to the region of chromosome 3 associated with renal cell carcinoma. Nature 332: 268-269, 1988. PubMed: 2894613

Ulf A Rapp, personal communication

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