黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pTK-HSV-BP2 plasmid in Escherichia coli B177845
pTK-HSV-BP2 plasmid in <em>Escherichia coli</em>
pTK-HSV-BP2 plasmid in <em>Escherichia coli</em>
規格:
價格:詢價
編號:B177845
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pTK-HSV-BP2 plasmid in Escherichia coli
商品貨號
B177845
Designations
pTK-HSV-BP2 plasmid in Escherichia coli
GenBank Number

U02031

培養基
Species
Homo sapiens, human
Depositors
JL Goldstein
Applications
in suitable host, produces protein sterol regulatory element binding transcription factor 2 sterol regulatory element-binding protein 2
Vector
Name of vector: pTK-HSV
Intact vector size: 5.8
Type of vector: phagemid
Vector end: BspDI
Vector end: XbaI
Cloning sites: BspDI XbaI
Construction: pcDNA3
Host range: mammalian cells; Escherichia coli


Features (with orientation and position when available):
replicon: pMB1
marker(s): neoR, ←
replicon: f1, ←
terminator: bGH polyadenylation
restriction site: XbaI
restriction site: BspDI
epitope tag: HSV
promoter: HSV TK, ←
marker(s): ampR, →
Insert
Insert size (kb): 4.2
Gene: sterol regulatory element binding transcription factor 2, SREBP2
DNA: cDNA
Source: human
Genbank accession : U02031
Nucleotides: Nucleotides 1-4093 of the insert correspond to nucleotides 156-4249 of U02031
Insert Size (kb)
4.2
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Freeze dried E. coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): BspDI/XbaI--5.8, 4.2; EcoRI--6.8, 3.0; HindIII--9.0; 0.8.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--1.08; HindIII--1.12; BglII--1.70; EcoRI--2.45
This construct allows the exogenously expressed SREBF2 to be cleaved in a sterol-regulated fashion after transfection into 293 cells (ATCC CRL-1573).
The amino terminus was truncated at amino acid 14 and fused to the HSV tag of the vector.
References

Hua X, et al. Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection. J. Biol. Chem. 270: 29422-29427, 1995. PubMed: 7493979

Joseph L Goldstein, personal communication

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479
  • 曹經理
    手機號：18662422060
    QQ：3671869992