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pMTK116
pMTK116
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B177906
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMTK116
商品貨號(hào)
B177906
Designations
pMTK116
Species
Mus musculus, mouse
培養(yǎng)基
Depositors
FH Ruddle
Vector
Construct size (kb): 0.0
Insert
DNA: genomic
Insert lengths(kb): 36.0
Tissue: liver
Gene product: thymidine kinase 1, soluble [Tk1]
Insert Size (kb)
36.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): SalI--12.0, 9.0, 8.0 doublet, 3.65; XhoI--41, 9.4; ClaI--35, 15; HindIII--23, 12, 5.5, 4.4; SalI/XhoI--9.0, 8.0, 7.5, 6.2, 5.5, 4.9, 3.35.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Insert includes the entire coding sequence as well as 5' and 3' flanking sequences on a 22 kb HpaI fragment. Promoter is either within or flanking the 5' end of the ApaI/XhoI fragment. Gene spans about 11 kb.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Lin PF, et al. Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences. Mol. Cell. Biol. 5: 3149-3156, 1985. PubMed: 3018504

Fiorentino L, et al. Assignment of the interleukin-2 locus to mouse chromosome 3. Genomics 5: 651-653, 1989. PubMed: 2613246

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