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pBUT
pBUT
規格:
價格:詢價
編號:B178043
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBUT
商品貨號
B178043
Designations
pBUT
GenBank Number

M16451

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 2.779999971389771
Insert
DNA: cDNA
Insert lengths(kb): 0.1840000003576279
Tissue: placenta
Gene product: creatine kinase, brain(creatine kinase B) [CKB]
Alleles: D1, D2, D1, D2
Insert Size (kb)
0.184
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.0, 0.2; HindIII--2.9; PstI--2.9; BamHI--2.9; EcoRI/HindIII--2.7, 0.2.
Constructed from the 184 bp HaeIII fragment (nt 1150 to nt 1335) in the 3' untranslated region which is specific to B creatine kinase and does not hybridize to M creatine kinase.
Hybridizes to mRNA from primary cultures of rat cardiac myocytes which express B creatine kinase.
EcoRI linkers were added during construction.
References

Dionne FT, et al. Ncol RFLP in human brain creatine kinase gene (CKBB). Nucleic Acids Res. 19: 195, 1991. PubMed: 1672741

Villarreal-Levy G, et al. Human creatine kinase: isolation and sequence analysis of cDNA clones for the B subunit, development of subunit-specific clones and determination of gene copy number. Biochem. Biophys. Res. Commun. 144: 1116-1127, 1987. PubMed: 3034271

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