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Distribution host: Escherichia coli DH5alpha

Size (kb): 5.314

DESCRIPTION OF VECTOR:
Intact vector size: 5.314
Type of vector: plasmid
Cloning sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV PstI SmaI BamHI SpeI
XbaI BstXI SacII SacI
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI BstXI SacII SacI
Construction: pBBR1MCS, tmpR
Host range: broad host range; Burkholderia pseudomallei; Burkholderia
cepacia; Escherichia coli
Features (with orientation and position when available):
coding sequence: MOB, <-, 5148-799
coding sequence: REP, ->, 1793-2455
MCS: SacI...KpnI, ->, 3220-3322
marker(s): tmpR, ->, 4387-4623

Vector: pBBR1Tp (plasmid)

Construction: pBBR1MCS, tmpR

Marker(s):tmpR

Construct size (kb): 5.314

Features: marker(s): tmpR
MCS: SacI...KpnI
coding sequence: MOB
coding sequence: REP

cloning vector

mobilizable vector

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): PstI--5.4; EcoRI--4.0, 0.64, 0.8; PvuII--2.4, 1.35, 1.1, 0.4

Plasmid is compatible with multiple replicons and can be transferred to other gram-negative bacteria by conjugation when the RK2 transfer functions are provided in trans.

Broad host range cloning vector allowing blue/white color detection of recominants and carrying the trimethoprim resistance gene as a selectable marker.

Temperature: 37.0°C