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Human coxsackievirus A3

規(guī)格:

價(jià)格:詢價(jià)

編號(hào):B178244

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Human coxsackievirus A3

商品貨號(hào)

B178244

Classification

Picornaviridae, Enterovirus, Enterovirus A

Agent

Human coxsackievirus A3

培養(yǎng)基

Strain

Olson

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 mL per vial

Storage Conditions

-70°C or colder

Images

Comments

Derived by adaptation from human amnion cell product ATCC^® VR-163™ to RD cells (ATCC^® CCL-136™). ATCC^® VR-163™ and ATCC^® VR-1799™ have not been compared for sequence or infectivity.

Effect on Host

CPE, rounding and sloughing

Recommended Host

RD (ATCC CCL-136)

Growth Conditions

Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days

Name of Depositor

HA Wenner, University of Kansas Medical Center; ATCC

Source

Stool of 2 year old with nuchal rigidity and hamstring muscle spasm, New York, 1948

References

Sickles GM, Dalldorf G. Serologic differences amoung strains of the coxsackie group of viruses. Proc. Soc. Exp. Biol. Med. 72(1): 30, 1949. PubMed: 15391667

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