For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector
Construct information: Total size of construct (kb): 4.2 Marker: ampR Promoter giving the sense strand: T3 Promoter giving the antisense strand: T7 Excise insert: XhoI + NotI
Vector information: Vector: pBluescript II KS+ Vector type: phagemid Vector size (kb): 2.961 Markers: ampR Vector end (5’): XhoI Vector end (3’): NotI
Insert
Insert size (kb): 1.32 Gene: pheB Source:Bacillus subtilis DNA: genomic Genbank accession: M24537 (Nucleotides 1-1318 of the insert correspond to nucleotides 2017-3334 of M24537) Insert end (5’): XhoI linker Insert end (3’): BamHI/poly(A)/NotI linker
Insert Size (kb)
1.320
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Frozen glycerol stock of ?E. coli containing the phagemid
Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 2017 - 3334 of Genbank M24537.
The insert contains the following restriction sites (approximate kb from the 5' end): XmnI--0.36; AvaI--0.89; EcoRI--1.05.
Restriction digests of the clone give the following sizes (kb): NotI--4.4; NotI/XhoI--3.0, 1.4; XhoI--4.4.
