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pWS7
pWS7
規(guī)格:
價格:詢價
編號:B178341
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pWS7
商品貨號
B178341
Designations
pWS7
GenBank Number

X14218

培養(yǎng)基
Species
Wound tumor virus
Depositors
DL Nuss
Vector
Construct size (kb): 4.590000152587891
Insert
DNA: cDNA
Insert lengths(kb): 1.730000019073486
Gene product: segment S7, core protein P6
Insert Size (kb)
1.730
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains an internal AccI site.
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.6; EcoRI--4.5; HindIII--4.5; PstI--4.5; XbaI--4.5.
Linearize the plasmid with SalI for in vitro transcription.
This is a full-length clone of virus segment S7.
The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1.
Classification
Phytoreovirus
References

Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991

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