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pSV5-dhfr
pSV5-dhfr
規格:
貨期:
編號:B178377
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pSV5-dhfr
商品貨號 B178377
Designations pSV5-dhfr
Depositors P Berg
Biosafety Level 1
Vector Information
Size (kb): 9.30
Vector: pSV5-dhfr (plasmid)
Promoters: Promoter SV40
Construction: pBR322, SV40, mouse
Marker(s):ampR,mtxR
Construct size (kb): 9.30
Features: marker(s): ampR, mtxR
other: polyoma large T antigen
promoter: SV40
replicon: pMB1
Applications
expression vector
shuttle vector
Comments
Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture strains.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398

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