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Primary Subcutaneous Pre-adipocytes; Normal, Human
Primary Subcutaneous Pre-adipocytes; Normal, Human
規格:
價格:
編號:B178456
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Primary Subcutaneous Pre-adipocytes; Normal, Human
商品貨號
B178456
Organism
Homo sapiens, human
Tissue
Mature adipose tissue
培養基
Morphology
Spindle-shaped, fibroblast-like
Growth Properties
Adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
Adult
Gender
Lot-specific
Ethnicity
Lot-specific
Applications
Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines
Product Format
frozen 1 mL
Storage Conditions
-130°C or below
Comments
ATCC® Human Pre-Adipocytes are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Complete Growth Medium
  1. Obtain one Fibroblast Growth Kit-Low Serum from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. 
  3. Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.

    6. Table 1. Fibroblast Growth Kit-Low Serum Components (ATCC® PCS-201-041)

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      Component 
                                              
                                    
                                                                    
                                                                                                                                                                                                
      
                                    
                                    
      
                                        
      
                                            Subculturing                                        
      
                                        
      
                                            
      Volume 
                                              
      
                                    
      
                                                                                                                                                                    
                                    
      
                                        
      
                                            Volume                                        
      
                                        
      
                                            
      Final Concentration 
                                              
      
                                    
      
                                                                    
      
                                                                                                                                                                                                
      
                                    
                                    
      
                                        
      
                                            Cells per Vial                                        
      
                                        
      
                                            
      rh FGF β 
                                              
      
                                    
      
                                                                                                                                                                    
                                    
      
                                        
      
                                            Sterility Tests                                        
      
                                        
      
                                            
      0.5 mL 
                                              
      
                                    
      
                                                                    
      
                                                                                                                                                                                                
      
                                    
                                    
      
                                        
      
                                            Viral Testing                                        
      
                                        
      
                                            
      5 ng/mL 
                                              
      
                                    
      
                                                                                                                                                                    
                                    
      
                                        
      
                                            Viability                                        
      
                                        
      
                                            
      L-glutamine 
                                              
      
                                    
      
                                                                    
      
                                                                                                                                                                                                
      
                                    
                                    
      
                                        
      
                                            C of A                                        
      
                                        
      
                                            
      18.75 mL 
                                              
      
                                    
      
                                                                    
      
                                                                                                                                                            
                            
                        
                        
                        
                        
      
                            

      

      
	
        
			
      

      
			

                        
      
                        
      
                            
      
                                
        
                                    
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