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Size (kb): 8.6000003814697270

Vector: pLTRdhfr26 (plasmid)

Promoters: Promoter Ha-SV LTR

Construction: pBR322, pDHFR26, HaSV clone H-1

Marker(s):ampR,mtxR (Dhfr)

Construct size (kb): 8.600000381469727

Features: marker(s): ampR, mtxR
promoter: Ha-SV LTR
replicon: pMB1

contains sequence dihydrofolate reductase

expression vector

integrating vector

shuttle vector

vector permitting positive selection for integration

Restriction digests of the clone give the following sizes (kb): BamHI--8.6, 1.36; EcoRI--9.4; HindIII--9.4.

Transfected methotrexate-resistant NIH 3T3 cells carried double minute chromosomes that appeared to contain DNA acquired during transfection.

Can be used in cotransfection experiments to generate a selectable methotrexate-resistant phenotype.

A 3.3 kb EcoRI/BamHI fragment of HaSV clone H-1 (including the LTR) was cloned into EcoRI/BamHI-digested pBR322. A PstI fragment with mouse Dhfr (with BamHI linkers) was then cloned into the BamHI site of the construct.

Temperature: 37.0°C