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Size (kb): 6.7030000686645510

Vector: pSP1 (phagemid)

Promoters: Promoter T3

Construction: pRS305 (ATCC 77140)

Marker(s):LEU2,ampR

Construct size (kb): 6.703000068664551

Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac, T3, T7
replicon: ars1
replicon: f1
replicon: pMB1

shuttle vector

vector containing primer sites useful for sequencing

vector for generating nested deletions of cloned inserts

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): KpnI--5.0, 1.8; SstI--6.7; HindIII--6.7.

Shuttle vector transforming S. pombe at high efficiency. Suitable for generating nested deletions, DNA sequencing, and producing riboprobes. Permits visual detection of recombinants by lacZalpha complementation.

Constructed from pRS305 by inserting a 1.2 kb EcoRI fragment containing ars1 into the AatII site. The NdeI site in ars1 was removed.

The order of the major features (with directions of transcription) is: < LEU2 - f1 - < lacZ' - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1.

Temperature: 37.0°C

component of:ATCC 87686