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pSP1

規(guī)格:

價(jià)格:詢(xún)價(jià)

編號(hào):B178767

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱(chēng)

pSP1

商品貨號(hào)

B178767

Designations

pSP1

Depositors

G Cottarel

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 6.7030000686645510

Vector: pSP1 (phagemid)

Promoters: Promoter T3

Construction: pRS305 (ATCC 77140)

Marker(s):LEU2,ampR

Construct size (kb): 6.703000068664551

Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac, T3, T7
replicon: ars1
replicon: f1
replicon: pMB1

Applications

shuttle vector

vector containing primer sites useful for sequencing

vector for generating nested deletions of cloned inserts

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Comments

Restriction digests of the clone give the following sizes (kb): KpnI--5.0, 1.8; SstI--6.7; HindIII--6.7.

Shuttle vector transforming S. pombe at high efficiency. Suitable for generating nested deletions, DNA sequencing, and producing riboprobes. Permits visual detection of recombinants by lacZalpha complementation.

Constructed from pRS305 by inserting a 1.2 kb EcoRI fragment containing ars1 into the AatII site. The NdeI site in ars1 was removed.

The order of the major features (with directions of transcription) is: < LEU2 - f1 - < lacZ' - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Cottarel G, et al. Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr. Genet. 23: 547-548, 1993. PubMed: 8319314