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IB800
IB800
規(guī)格:
價格:
編號:B178931
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
IB800
商品貨號
B178931
Designations
IB800
GenBank Number

T03725

培養(yǎng)基
Species
Homo sapiens, human
Depositors
JM Sikela
Applications
The primers can also be used to generate sequence from the 3' or 5' ends, respectively.
Vector
Construct size (kb): 4.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S8933E
Gene name: DNA Segment, expressed
Contains complete coding sequence?: U
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5' end: Modification: HindIII
Insert 3' end: Modification: NotI
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: T03725
Insert lengths(kb): 1.5
Tissue: infant brain, 3 months old (from Bento Soares)
Gene product: DNA Segment, expressed [D0S8933E]
Insert Size (kb)
1.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; ClaI--4.6; HindIII/NotI-- 3.1, 0.9, 0.6; PvuII--2.8, 1.3, 0.31; ScaI--4.6; EcoRI--4.6.
Derived from a directionally cloned library with an average insert size of 1.7 kb.
Inserts can be amplified with the M13 universal (forward) and reverse primers. The primers can also be used to generate sequence from the 3' or 5' ends, respectively.
References

Khan AS, et al. Single pass sequencing and physical and genetic mapping of human brain cDNAs. Nat. Genet. 2: 180-185, 1992. PubMed: 1345165

Soares MB, et al. Construction and characterization of a normalized cDNA library. Proc. Natl. Acad. Sci. USA 91: 9228-9232, 1994. PubMed: 7937745

Marcelo B Soares, personal communication

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