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HuCK
HuCK
規格:
價格:
編號:B178948
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HuCK
商品貨號
B178948
Designations
HuCK
Species
Homo sapiens, human
培養基
Depositors
P Leder
Vector
Construct size (kb): 6.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.5
Gene product: immunoglobulin kappa constant region [IGKC]
Alleles: A1, A2
Insert Size (kb)
2.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 2.7; HindIII--6.6; PstI--6.6; BamHI--6.6; PvuII--4.4, 3.2.
The insert contains HaeIII, SacI (2), MboII, AccI, and HphI sites.
The insert includes the complete exon for the constant region plus 5' and 3' flanking sequences.
The polymorphic SacI site is located 3' to the SacI site in the coding sequence of the kappa constant region.
Enzyme(s) not detecting polymorphism: ApaI, AvaII, BamHI, BclI, BglII, BstEII, EcoRI, EcoRV, HincII, HindIII, HinfI, KpnI, MboI, MspI, PstI, PvuII, TaqI, XbaI (tested on 7 unrelated Caucasians).
References

Field LL, et al. SacI RFLP recognized by a human immunoglobulin kappa light chain constant region probe. Nucleic Acids Res. 15: 3942, 1987. PubMed: 2884643

Hieter PA, et al. Evolution of human immunoglobulin kappa J region genes. J. Biol. Chem. 257: 1516-1522, 1982. PubMed: 6276389

Weir L, Leder P. Structure and expression of a human subgroup II immunoglobulin kappa gene. Nucleic Acids Res. 14: 3957-3970, 1986. PubMed: 3086847

Hieter PA, et al. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell 22: 197-207, 1980. PubMed: 6775818

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