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AR17
AR17
規(guī)格:
價格:
編號:B179037
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
AR17
商品貨號
B179037
Designations
AR17
GenBank Number

X83543

培養(yǎng)基
Species
Homo sapiens, human
Depositors
MV Schiaffino
Applications
contains sequence apical protein, Xenopus laevis-like
Vector
Construct size (kb): 6.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: APXL
Gene name: apical protein, Xenopus laevis-like
Contains complete coding sequence?: N
Tissue: retina, adult
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.223
Cross references: DNA Seq. Acc.: X83543
Nucleotides 1-3223 of the insert correspond to
nucleotides 403-3625 of X83543.
Insert lengths(kb): 3.223000049591065
Tissue: retina, adult
Gene product: apical protein, Xenopus laevis-like [APXL]
Target Gene: apical protein, Xenopus laevis-like
Insert Size (kb)
3.223
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 3.0; BamHI--4.7, 1.5; HindIII--3.7, 2.5.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.0; BglI--2.3, 2.45; HindIII--2.65; SacI--0.95, 2.5, 3.0; SalI--0.05; PstI--1.05, 1.75, 1.8; PvuI--0.95; PvuII--1.0, 1.25, 2.1; XhoI--1.35, 2.9, 2.95.
Contains a portion of the coding sequence, overlapping AR16 (ATCC 99508) at the 5' end and AR24 (ATCC 99512) at the 3' end.
The coding region of GenBank X83543 corresponds to nt 91-4941.
References

Schiaffino MV, et al. Cloning of a human homologue of the Xenopus laevis APX gene from the ocular albinism type 1 critical region. Hum. Mol. Genet. 4: 373-382, 1995. PubMed: 7795590

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