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SU-DHL-8
SU-DHL-8
規(guī)格:
價格:詢價
編號:B179053
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SU-DHL-8
商品貨號
B179053
Organism
Homo sapiens, human
Tissue
lymph node; derived from metastatic site: peritoneal effusion
培養(yǎng)基
Cell Type
B lymphocyte
Product Format
frozen
Morphology
lymphoblast-like
Culture Properties
suspension, grow as single cells and small clusters
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Large cell lymphoma
Age
59 years
Gender
Male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Images
CRL-2961 Micrograph
Clinical Data
59 years
Caucasian
Male
Receptor Expression
Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed)
Genes Expressed
Ig +; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa-
Comments

The depositor states that this cell line is negative for Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
This cell line is negative for Canadida ingestion.
This is a null cell line (absence of all known markers) based on the classification of diffuse histiocytic lymphomas.
These cells exhibit no response to LY294002-induced apoptosis.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium. Subculture the cells when cell density is ~ 1.0 x 106 to 2.5 x 106 cells/mL for higher viability. An inoculum of 1.0 x 105 to 4.0 x 105 cells/mL is recommended.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended.
Medium renewal: Every 2 to 4 days (depending on cell density)
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 11, 13
D13S317: 11, 13
D7S820: 8
D16S539: 12
CSF1PO: 11, 12
vWA: 15, 19
THO1: 6, 9
TPOX: 8
Amelogenin: X
Year of Origin
1978
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

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