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pTrec2 plasmid in E. coli
pTrec2 plasmid in E. coli
規(guī)格:
價(jià)格:詢價(jià)
編號:B179117
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pTrec2 plasmid in E. coli
商品貨號
B179117
Designations
pTrec2 plasmid in E. coli
GenBank Number

M77094

培養(yǎng)基
Species
Bos taurus, cow
Depositors
S Zozulya, L Stryer
Vector
Construct size (kb): 4.1
Insert
DNA: cDNA
Insert lengths(kb): 0.7
Tissue: retina
Gene product: recoverin
Insert Size (kb)
0.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI/NdeI--3.2, 0.78; BglII--4.1; EcoRI--3.7, 0.31; XbaI--4.1.
The 5' nt of the insert (assuming it to be the first C in the NdeI site CATATG) corresponds to nt 25 in the sequence record. However the next two nt were changed by PCR from CC in the wild type cDNA to AT to create the NdeI site.
Contains the complete coding sequence and 0.15 kb of the 3' untranslated region.
References

Ray S, et al. Cloning, expression, and crystallization of recoverin, a calcium sensor in vision. Proc. Natl. Acad. Sci. USA 89: 5705-5709, 1992. PubMed: 1385864

Dizhoor AM, et al. Recoverin: a calcium sensitive activator of retinal rod guanylate cyclase. Science 251: 915-918, 1991. PubMed: 1672047

Sergey Zozulya, personal communication

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