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pBfm3
pBfm3
規(guī)格:
價(jià)格:
編號:B179142
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBfm3
商品貨號
B179142
Designations
pBfm3
Species
Homo sapiens, human
培養(yǎng)基
Depositors
KE Davies
Vector
Construct size (kb): 6.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 3.5
Tissue: fetal muscle
Gene product: utrophin (homologous to dystrophin)(utrophin) [DMDL]
Insert Size (kb)
3.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5, 2.7; PstI--3.1, 3.1; HindIII/EcoRI--2.7, 1.9, 1.7; HindIII--4.3, 1.9.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--1.9; PstI--3.2; XbaI--3.2.
The complete probe detects genomic EcoRI fragments of the following sizes (kb): human--15, 6.5, 3.7, 3.0, 1.8, 1.0, 0.3; mouse--16, 9.0, 4.0, 3.8, 1.0; Chinese hamster--7.5, 7.3, 2.0.
The 5' 1.9 kb EcoRI/HindIII fragment from the insert, that contains the 3' coding region of the transcript, detects human genomic PstI fragments of the following sites (kb): 19, 6.8, 5.0, 3.7, 3.0, and 1.8.
The 3' 1.6 kb HindIII/EcoRI fragment from the insert, that contains 3' untranslated sequences, detects genomic PstI fragments of the following sizes (kb): human--1.8; mouse--15; rat--7.0; hamster--7.0.
Detects a transcript of 13 kb that is expressed ubiquitously.
References

Love DR, et al. An autosomal transcript in skeletal muscle with homology to dystrophin. Nature 339: 55-58, 1989. PubMed: 2541343

Love DR, et al. Tissue distribution of the dystrophin-related gene product and expression in the mdx and dy mouse. Proc. Natl. Acad. Sci. USA 88: 3243-3247, 1991. PubMed: 2014247

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