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AF3
AF3
規格:
貨期:
編號:B179145
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 AF3
商品貨號 B179145
Designations AF3
GenBank Number

M10157

Species Homo sapiens, human
Applications
Codominant segregation of the PvuII RFLP has been observed in 10 families (100 individuals).
A 619 bp HincII/EcoRI fragment must be used to detect the RFLP. There is low background at a wash stringency of 0.2X SSPE at 60C. This probe also detects non-polymorphic sequences from chromosome 2.
Vector
Construct size (kb): 3.700000047683716
Insert
DNA: cDNA
Insert lengths(kb): 1.057999968528748
Tissue: hepatoma
Gene product: fucosidase, alpha-L- 1, tissue( Fucosidase, alpha-L-1, tissue, alpha-L-fucosidase) [FUCA1]
Alleles: A1, A2, B2, B1
Insert Size (kb) 1.058
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.0; PstI--3.7; HindIII--3.7; BamHI--3.7; XbaI--3.7.
Detects genomic EcoRI fragments of 5.5, 3.8, 3.0, 2.6, 2.0, and 0.9 kb in the human; 8.0 and 0.9 kb in the mouse; 6.5 and 0.9 kb in the rat; and 10.0, 6.0, 5.0, and 3.0 in the Chinese hamster.
Excluding the linker sequences, the insert contains 1043 bp coding for alpha-fucosidase (80% of the processed enzyme).
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Codominant segregation of the PvuII RFLP has been observed in 10 families (100 individuals).
A 619 bp HincII/EcoRI fragment must be used to detect the RFLP. There is low background at a wash stringency of 0.2X SSPE at 60C. This probe also detects non-polymorphic sequences from chromosome 2.
References

Guazzi S, et al. Heterogeneity of mRNA expression in Italian fucosidosis patients. Hum. Genet. 82: 63-66, 1989. PubMed: 2565868

Daly ME, Perry DJ. BclI RFLP in fucosidase pseudogene (FUCA1P). Nucleic Acids Res. 18: 5583, 1990. PubMed: 1977129

Fukushima H, et al. Molecular cloning of a cDNA for human alpha-l-fucosidase. Proc. Natl. Acad. Sci. USA 82: 1262-1265, 1985. PubMed: 2983333

Fowler ML, et al. Chromosome 1 localization of the human alpha-l-fucosidase structural gene with a homologous site on chromosome 2. Cytogenet. Cell Genet. 43: 103-108, 1986. PubMed: 3780313

Darby JK, et al. PvuII RFLP at the human chromosome 1 alpha-l-fucosidase gene locus (FUCA1). Nucleic Acids Res. 14: 9543, 1986. PubMed: 2879270

Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294

Barbara Weiffenbach, personal communication

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