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pYNH37.3
pYNH37.3
規(guī)格:
價(jià)格:
編號(hào):B179416
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pYNH37.3
商品貨號(hào)
B179416
Designations
pYNH37.3
Species
Homo sapiens, human
培養(yǎng)基
Depositors
RL White, Y Nakamura
Applications
RFLPs were observed under normal hybridization stringency.
Vector
Construct size (kb): 4.25
Insert
DNA: genomic
Insert lengths(kb): 1.5
Gene product: DNA Segment, single copy [D17S28]
Insert Size (kb)
1.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.25; HindIII--4.25; EcoRI/HindIII--2.85, 1.40; BamHI--4.25; PstI--4.25.
pYNZ22 (ATCC 57574) and pYNH37 (ATCC 57568) map to the same 30 kb SfiI fragment.
The TaqI polymorphism demonstrates codominant segregation in more than 50 families.
RFLPs were observed under normal hybridization stringency. Washes at 0.1X SSC, 0.1% SDS, 65C.
The six-base cutters EcoRI, HindIII, BamHI, PvuII, PstI and BglII do not adequately resolve the polymorphism.
References

vanTuinen P, et al. Molecular detection of microscopic and submicroscopic deletions associated with Miller-Dieker syndrome. Am. J. Hum. Genet. 43: 587-596, 1988. PubMed: 3189330

Schwartz CE, et al. Detection of submicroscopic deletions in band 17p13 in patients with the Miller-Dieker syndrome. Am. J. Hum. Genet. 43: 597-604, 1988. PubMed: 2903661

Baker SJ, et al. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244: 217-221, 1989. PubMed: 2649981

Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Barker D, et al. Gene for von Recklinghausen neurofibromatosis is in the pericentromeric region of chromosome 17. Science 236: 1100-1102, 1987. PubMed: 3107130

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pYNH37.3 on chromosome 17p [D17S28]. Nucleic Acids Res. 16: 782, 1988. PubMed: 2893350

Peter J O'Connell, personal communication

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