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LL21NL03
LL21NL03
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B179500
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
LL21NL03
商品貨號(hào)
B179500
Designations
LL21NL03
Species
Homo sapiens, human
培養(yǎng)基
Depositors
JA Garnes
Origin
mouse/human WAV-17 hybrid cell line
Applications
Derivation and characterization of the WAV-17 cell line were described in J. Exp.
Vector
Tissue: mouse/human WAV-17 hybrid cell line
Chromosome: 21
DNA: genomic
Digest: Partial
Insert size range: 15 kb average
Genome Equivalent: 50.0
Coverage: Clones for "complete" coverage: 3870.0
Media
ATCC® Medium 1592: SM buffer
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: frozen (volume: 0.2 ml)
Comments
Purity (human clones): 82%. Contaminants (mouse): 10.5%.
Verified to have an average insert size of 15 kb based on NotI digestion of DNA purified from the library.
Chromosomes for library construction were isolated using flow sorting.
Genome equivalents (average redundancy) were calculated by correcting for purity and assuming 15 kb average inserts and 49.2 Mb per chromosome 21.
Derivation and characterization of the WAV-17 cell line were described in J. Exp. Med. 137: 317-330, 1973; Proc. Natl. Acad. Sci. USA 81: 5504-5508, 1984 and Genomics 8: 614-622, 1990.
References

Trask BJ, et al. Characterization of somatic cell hybrids by bivariate flow karyotyping and fluorescence in situ hybridization. Somatic Cell Mol. Genet. 17: 117-136, 1991. PubMed: 2011792

Dunn IS, Blattner FR. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers. Nucleic Acids Res. 15: 2677-2698, 1987. PubMed: 3031608

Jeffrey A Garnes, personal communication

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