Restriction digests of the clone give the following sizes (kb): EcoRI--2.6, 1.4; HindIII--3.3, 0.7; PvuII--2.4, 0.8. The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.44; HindIII--0.60; XhoI--0.88; XmnI--1.16. The strain was originally isolated in New York State. Contains the complete coding sequence for the coat protein (nt 298-954) and the complete intergenic region. |