Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATalpha ura3-52 lys2 ade1 ade2 his7 trp1-delta1
(The lys2 mutation in YP80 and YP148 complement the lys2-801 allele in YNN282 (ATCC 204662).), (Chromosome VII is split into 2 fragments at RAD2. The larger fragment has URA3 + pBR sequences proximal to RAD2 and migrates between bands 10b and 11 in OFAGE gels; the smaller fragment has TRP1 + CEN4 + pBR + sequences distal to RAD2 and migrates as a mini chromosome band below Chromosome I.)
Preceptrol®
no
Comments
Mapping strains: mapping by chromosome separation (AB10)
Medium
ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Name of Depositor
YGSC
Special Collection
Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--P. Hieter and R. Davis
References
The lys2 mutation in YP80 and YP148 complement the lys2-801 allele in YNN282 (ATCC 204662).
Chromosome VII is split into 2 fragments at RAD2. The larger fragment has URA3 + pBR sequences proximal to RAD2 and migrates between bands 10b and 11 in OFAGE gels; the smaller fragment has TRP1 + CEN4 + pBR + sequences distal to RAD2 and migrates as a mini chromosome band below Chromosome I.
The karyotype of YP148 is the same as YP80 (ATCC 204652) except that chromosome VII is split into 2 fragments at RAD2. The larger fragment has URA3 + pBR sequences proximal to RAD2 and migrates between bands 10b and 11 in OFAGE gels; the smaller fragment has TRP1 + CEN4 + pBR + sequences distal to RAD2 and migrates as a mini chromosome band below chromosome I.
The lys2 mutation in YP80 and YP148 complement the lys2-801 allele in YNN282 (ATCC 204662).
Chromosome VII is split into 2 fragments at RAD2. The larger fragment has URA3 + pBR sequences proximal to RAD2 and migrates between bands 10b and 11 in OFAGE gels; the smaller fragment has TRP1 + CEN4 + pBR + sequences distal to RAD2 and migrates as a mini chromosome band below Chromosome I.
