Wild type strains: yeast strains used to make gene pool libraries (AA3)
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
HO ade2-1 lys2-1 trp5-2 leu2-1 can1-100 ura3-1 met4-1 leu1
RefNasmyth KA, Tatchell K. The structure of transposable yeast mating type loci. Cell 19: 753-764, 1980. PubMed: 6244896
Preceptrol®
no
Ploidy
haploid
Comments
Wild type strains: yeast strains used to make gene pool libraries (AA3)
Used to make the YEp13 yeast gene pool for transformation
Morphology
After 3 days at 25°C growth is butyrous and light cream colored.? Cells are globose, ovoidal or elongate, (3.0-8.0) x (5.0-10.0) μm, and are isolated or in small groups.? Sediment is present after one month.
Medium
ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Sequenced Data
No DNA sequencing was performed in house on this product.
Name of Depositor
YGSC
Special Collection
Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--K.A. Nasmyth and K. Tatchell
References
Nasmyth KA, Tatchell K. The structure of transposable yeast mating type loci. Cell 19: 753-764, 1980. PubMed: 6244896
Nasmyth KA, Tatchell K. The structure of transposable yeast mating type loci. Cell 19: 753-764, 1980. PubMed: 6244896