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pVE328 [MB5331]
pVE328 [MB5331]
規格:
價格:詢價
編號:B179697
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pVE328 [MB5331]
商品貨號
B179697
Designations
pVE328 [MB5331]
Depositors
Merck & Co., Inc.
培養基
U.S. Patent
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Vector Information
Construct size (kb): 7.5
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--7.2, 0.3; PstI--3.4, 3.25, 0.95; HindIII--7.5.
pVE328 is available as ATCC 67698 and ATCC 68408.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Cosmid shuttle vector permitting packaging both as bacteriophage lambda and TG1 virions.
The order of the major features in this plasmid is: pVE1 ori - BalI - tsrR - SstI - lambda cos - lamda cos - HindIII - TG1 cos - XbaI/MCS/PstI.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

MacNeil DJ. Process for the incorporation of DNA molecules into microorgansims with methyl-specific restriction systems. US Patent 5,194,387 dated Mar 16 1993

Gewain KM, et al. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use. US Patent 5,252,474 dated Oct 12 1993

MacNeil DJ. Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J. Bacteriol. 170: 5607-5612, 1988. PubMed: 3056907

Shipped
freeze-dried
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