pUCSV40-T302-1 [molecular clone of Simian virus 40, SV-40]
商品貨號(hào)
B179948
Designations
pUCSV40-T302-1 [molecular clone of Simian virus 40, SV-40]
Species
Simian virus 40, Simian virus 40
Depositors
JA Lednicky, JS Butel
Origin
SV40-T302-1 was isolated in CV-1 cells (ATCC CCL-70) from the brain of an SIV-infected juvenile Rhesus monkey. This is a clone of a unique brain isolate of SV40 (slowest growing SV40 natural isolate encountered at time of deposit.)
Isolation
SV40-T302-1 was isolated in CV-1 cells (ATCC CCL-70) from the brain of an SIV-infected juvenile Rhesus monkey. This is a clone of a unique brain isolate of SV40 (slowest growing SV40 natural isolate encountered at time of deposit.)
Applications
contains sequence
Vector
Construct size (kb): 7.842000007629395
DESCRIPTION OF VECTOR COMPONENT
??????? Type of vector:? E. coli
??????? Name of vector:? pUC-19
??????? Intact vector size (kb):? 2.686 kb
Insert
DNA: genomic
Insert lengths(kb): 5.156000137329102
Tissue: Rhesus monkey brain
Insert Size (kb)
5.156
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Description of cloned agent:
Name: SV40
Strain: T302-1
Classification: Papovavirus
Full length clone of SV40 strain T302
Brain tissue was minced in PBS, freeze-thawed three times to lyse the cells, then passed through a 0.45 mm filter. The filtrate was used to inoculate ATCC CV-1 cells (passage 34). The viral regulatory region of this construct is archetypal.
Classification
Polyomaviridae, Polyomavirus
References
Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685
