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h628 partial cDNA
h628 partial cDNA
規格:
價格:詢價
編號:B180138
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
h628 partial cDNA
商品貨號
B180138
Designations
h628 partial cDNA
GenBank Number

U29366

Z50115

培養基
Species
Homo sapiens, human
Depositors
P Malherbe
Vector
Construct size (kb): 5.349999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBC/CMV
Intact vector size: 4.700
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII
Polylinker sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII
Host range: eukaryotic cells; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1
promoter for expression: CMV, ->
other: rat preproinsulin II (rI2) intron, ->
terminator: SV40 polyadenylation signal, ->
marker(s): ampR
replicon: SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: THOP1
Gene name: thimet oligopeptidase 1
Contains complete coding sequence?: N
Tissue: neuroblastoma cell line IMR32
Type of DNA: cDNA
Insert end: Modification: blunted
Insert end: Modification: blunted
Insert size (kb): 0.628
Cross references: DNA Seq. Acc.: U29366
Nucleotides 1-628 of the insert correspond to
nucleotides 345-973 of U29366.
DNA Seq. Acc.: Z50115
Nucleotides 1-628 of the insert correspond to
nucleotides 385-1013 of Z50115.
Insert lengths(kb): 0.628000020980835
Tissue: neuroblastoma cell line IMR32
Gene product: thimet oligopeptidase 1(Thimet oligopeptidase) [THOP1]
Insert Size (kb)
0.628
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.6, 0.65; EcoRI--5.3; KpnI--4.7, 0.35.
Insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.16; StuI--0.47.
Corresponds to part of the coding region, which is nt 128-2197 of GenBank Z50115.
References

Thompson A, et al. Cloning and functional expression of a metalloendopeptidase from human brain with the ability to cleave a beta-APP substrate peptide. Biochem. Biophys. Res. Commun. 213: 66-73, 1995. PubMed: 7639763

Parichehr Malherbe, personal communication

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