| 產品名稱 |
h628 partial cDNA |
| 商品貨號 |
B180138 |
| Designations |
h628 partial cDNA |
| GenBank Number |
U29366
Z50115
|
| Species |
Homo sapiens, human |
| Depositors |
P Malherbe |
| Vector |
Construct size (kb): 5.349999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBC/CMV
Intact vector size: 4.700
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII
Polylinker sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII
Host range: eukaryotic cells; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1
promoter for expression: CMV, ->
other: rat preproinsulin II (rI2) intron, ->
terminator: SV40 polyadenylation signal, ->
marker(s): ampR
replicon: SV40
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: THOP1
Gene name: thimet oligopeptidase 1
Contains complete coding sequence?: N
Tissue: neuroblastoma cell line IMR32
Type of DNA: cDNA
Insert end: Modification: blunted
Insert end: Modification: blunted
Insert size (kb): 0.628
Cross references: DNA Seq. Acc.: U29366
Nucleotides 1-628 of the insert correspond to
nucleotides 345-973 of U29366.
DNA Seq. Acc.: Z50115
Nucleotides 1-628 of the insert correspond to
nucleotides 385-1013 of Z50115. Insert lengths(kb): 0.628000020980835 Tissue: neuroblastoma cell line IMR32 Gene product: thimet oligopeptidase 1(Thimet oligopeptidase) [THOP1] |
| Insert Size (kb) |
0.628 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.6, 0.65; EcoRI--5.3; KpnI--4.7, 0.35. Insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.16; StuI--0.47. Corresponds to part of the coding region, which is nt 128-2197 of GenBank Z50115. |
| References |
Thompson A, et al. Cloning and functional expression of a metalloendopeptidase from human brain with the ability to cleave a beta-APP substrate peptide. Biochem. Biophys. Res. Commun. 213: 66-73, 1995. PubMed: 7639763
Parichehr Malherbe, personal communication
|
