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Quantitative Synthetic Human immunodeficiency virus 1 (HIV-1) RNA
Quantitative Synthetic Human immunodeficiency virus 1 (HIV-1) RNA
規格:
價格:詢價
編號:B180498
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Quantitative Synthetic Human immunodeficiency virus 1 (HIV-1) RNA
商品貨號
B180498
Strain
Quantitative Synthetic Human immunodeficiency virus 1 (HIV-1) RNA
Applications
ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load and for other molecular biology applications.
培養基
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Specification range: 1 x 105 to 1 x 106 copies/μL
100 μL per vial with Biomatrica RNAstable
Storage Conditions
-70°C or colder
Intended Use
The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

Biomatrica Logo
Comments
Manufactured under ISO 13485 guidance

Preparation includes fragments from the 5' LTR, gag gene, pol gene (including protease, reverse transcriptase, and integrase regions), tat gene, rev gene, and nef gene.

This construct also includes clinically significant mutation profiles to allow compatibility with the CDC genotyping assay patent, including 41L, 67N, 70R, 103N, 181C, 215Y, and 90M. (Centers for Disease Control and Prevention. Real-time PCR point mutation assays for detecting HIV-1 resistance to antiviral drugs. US Patent 8,592,146 dated Nov. 26, 2013)

The following primers and probe can be used with this nucleic acid preparation RefHenrich TJ, et al. Low-level detection and quantitation of cellular HIV-1 DNA and 2-LTR circles using droplet digital PCR. J. Virol. Methods 186(1-2): 68-72, 2012. PubMed: 22974526:
Forward: TACTGACGCTCTCGCACC
Reverse: TCTCGACGCAGGACTCG
Probe: CTCTCTCCTTCTAGCCTC
Name of Depositor
ATCC
U.S. Patent
8,592,146
U.S. Patent Number
8,592,146
Special Collection
RNA
References

Henrich TJ, et al. Low-level detection and quantitation of cellular HIV-1 DNA and 2-LTR circles using droplet digital PCR. J. Virol. Methods 186(1-2): 68-72, 2012. PubMed: 22974526

Centers for Disease Control and Prevention. Real-time PCR point mutation assays for detecting HIV-1 resistance to antiviral drugs. US Patent 8,592,146 dated Nov. 26, 2013

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