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Human rhinovirus 1B
Human rhinovirus 1B
規格:
價格:詢價
編號:B180556
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Human rhinovirus 1B
商品貨號
B180556
Classification
Picornaviridae, Enterovirus, Human rhinovirus A
Agent
Human rhinovirus 1B
培養基
Strain
B632
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Genome Sequenced Strain

Human rhinovirus 1B, complete genome

Comments
Strain B632 is the prototype strain for Human rhinovirus 1B.
Effect on Host
CPE, rounding and sloughing
Recommended Host
H1HeLa (ATCC® CRL-1958™)
Growth Conditions
Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere rocking continuously. End adsorption by adding virus growth medium
Incubation: 2-7 days
Name of Depositor
National Institute of Allergy and Infectious Diseases, ATCC®
Source
Presumed from human throat washings. Treated ATCC® VR-1111™ to remove mycoplasma at ATCC®.
References

Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.

Hughes PJ, et al. The nucleotide sequence of human rhinovirus 1B: molecular relationships within the rhinovirus genus. J. Gen. Virol. 69 (PT1): 49-58, 1988. PubMed: 2826669

Taylor-Robinson D, Tyrrell DA. Serotypes of viruses (Rhinoviruses) isolated from common colds. Lancet 1: 452-454, 1962. PubMed: 13920011

Cross References

Nucleotide (GenBank) : D00239.1 Human rhinovirus 1B, complete genome

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