| 產(chǎn)品名稱 |
Human rhinovirus 1B |
| 商品貨號(hào) |
B180556 |
| Classification |
Picornaviridae, Enterovirus, Human rhinovirus A |
| Agent |
Human rhinovirus 1B |
| Strain |
B632 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Genome Sequenced Strain |
Human rhinovirus 1B, complete genome
|
| Comments |
Strain B632 is the prototype strain for Human rhinovirus 1B. |
| Effect on Host |
CPE, rounding and sloughing |
| Recommended Host |
H1HeLa (ATCC® CRL-1958™) |
| Growth Conditions |
Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere rocking continuously. End adsorption by adding virus growth medium
Incubation: 2-7 days |
| Name of Depositor |
National Institute of Allergy and Infectious Diseases, ATCC® |
| Source |
Presumed from human throat washings. Treated ATCC® VR-1111™ to remove mycoplasma at ATCC®. |
| References |
Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.
Hughes PJ, et al. The nucleotide sequence of human rhinovirus 1B: molecular relationships within the rhinovirus genus. J. Gen. Virol. 69 (PT1): 49-58, 1988. PubMed: 2826669
Taylor-Robinson D, Tyrrell DA. Serotypes of viruses (Rhinoviruses) isolated from common colds. Lancet 1: 452-454, 1962. PubMed: 13920011
|
| Cross References |
Nucleotide (GenBank) :
D00239.1
Human rhinovirus 1B, complete genome
|
