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pHUMNHE1
pHUMNHE1
規(guī)格:
價(jià)格:
編號(hào):B180665
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHUMNHE1
商品貨號(hào)
B180665
Designations
pHUMNHE1
GenBank Number

M81768

培養(yǎng)基
Species
Homo sapiens, human
Depositors
DG Warnock
Vector
Construct size (kb): 7.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: HindIII
Vector end: SmaI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->, 3-459
primer site: M13-20, ->
promoter for in vitro transcription: T7, ->
insert detection: lacZ', <-, 816-938
MCS: SacI...KpnI, ->, 657-759
promoter for in vitro transcription: T3, <-
primer site: reverse, <-
replicon: pMB1, 1032-1972
marker(s): ampR, <-, 1975-2832
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SLC9A1
Other gene symbols: APNH; NHE1
Gene name: solute carrier family 9 (sodium/hydrogen exchanger),
isoform 1 (antiporter, Na+/H+, amiloride sensitive)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 p36.1-p35
Tissue: kidney cortex
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: Modification: blunt ended
Insert size (kb): 4.0
Cross references: DNA Seq. Acc.: M81768
Insert lengths(kb): 4.0
Tissue: kidney cortex
Gene product: NA(+)/H(+) antiporter 1( Na+/H+ antiporter, Na+/H+ antiporter protein) [APNH]
Insert Size (kb)
4.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.2, 3.1; BamHI--3.4, 2.0, 1.5, 0.41; PstI--7.2; SacI--7.2; PvuII--2.7, 2.45, 1.10, 0.56, 0.28.
Constructed from a 520 bp HindIII/PstI PCR product and the large PstI/EcoRI fragment from HK-3.5.
The SmaI site was lost during cloning, and the vector SacI site was deleted.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.38, 2.3, 3.7; PstI--0.52; SacI--1.1; PvuII--2.1, 2.6, 2.9, 3.9.
References

Takaichi K, et al. Cloning, sequencing, and expression of Na+-H+ antiporter cDNAs from human tissues. Am. J. Physiol. 262: C1069-C1076, 1992. PubMed: 1314485

David G Warnock, personal communication

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