Comments
The 28-2-7 (ATCC CRL-2487) and 28-6-20 (ATCC CRL-2489) antibodies bind the V kappa 24 light chain idiotypic specificity of MOPC167.
Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibodies are specific for PC-binding proteins of VH1/V kappa 24 heavy and light chains of various isotypes. They do not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins.
These antibodies can be used as typing reagents for mouse IgA bearing the V kappa 24 idiotypic specificity.
They can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of the IgA PC specific MOPC167 myeloma.
