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pMyc3Pst
pMyc3Pst
規格:
貨期:
編號:B180737
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pMyc3Pst
商品貨號 B180737
Designations pMyc3Pst
GenBank Number

J02019

Species avian myelocytomatosis virus (type C)
Depositors J Bishop
Vector
Construct size (kb): 5.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 1.5
Gene product: avian myelocytomatosis viral (v-myc) oncogene homolog( avian myelocytomatosis viral (v-myc) oncogene homolog, proto-oncogene c-myc) [MYC]
Insert Size (kb) 1.5
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.1, 1.5; EcoRI--5.6; HindIII--5.6; BamHI--5.6.
Encodes from approximately amino acid 52 of myc portion of gag-myc polyprotein into region of homology with Pr-C RSV genome (1581), i.e. 158-1676. env sequences are from 1581-1676; region 450-700 is very GC-rich (GC/ATGC = 0.84).
References

Kochevar DT, et al. Low level amplification of c-sis and c-myc in a spontaneous osteosarcoma model. Cancer Lett. 53: 213-222, 1990. PubMed: 2208081

Vennstrom B, et al. Molecular cloning of the avian myelocytomatosis virus genome and recovery of infectious virus by transfection of chicken cells. J. Virol. 39: 625-631, 1981. PubMed: 6268847

Alitalo K, et al. Nucleotide sequence of the v-myc oncogene of avian retrovirus MC29. Proc. Natl. Acad. Sci. USA 80: 100-104, 1983. PubMed: 6296857

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