| Vector |
Construct size (kb): 4.900000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: OGDH
Genomic copy number: unique
Gene name: 2-oxo-glutarate dehydrogenase complex, (E1 component)
Contains complete coding sequence?: N
Localization: 7 p13-p11.2
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.6
Cross references: DNA Seq. Acc.: D10523; EST: EST06250
Nucleotides 1-341 of the insert correspond to
nucleotides 4141-3803 of D10523 with 95% identity.
DNA Seq. Acc.: D10523; EST: EST06251
Nucleotides 1-367 of the insert correspond to
nucleotides 2529-2895 of D10523 with 97% identity.
DNA Seq. Acc.: T08359; EST: EST06250; Primer: M13-21; THC
id: THC100746
DNA Seq. Acc.: T08360; EST: EST06251; Primer: M13 Reverse;
THC id: THC100746 Insert lengths(kb): 1.600000023841858 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: 2-oxo-glutarate dehydrogenase complex, (E1 component) [OGDH] |
