| 產(chǎn)品名稱 |
pfos-1 [p.fos-1] |
| 商品貨號 |
B180853 |
| Designations |
pfos-1 [p.fos-1] |
| Species |
Murine osteosarcoma virus |
| Depositors |
T Curran |
| Applications |
The 1.0 kb PstI fragment is usually used for the fos probe. |
| Vector |
Construct size (kb): 5.800000190734863 |
| Insert |
DNA: genomic Insert lengths(kb): 1.299999952316284 Gene product: FBJ murine osteosarcoma viral (v-fos) oncogene homolog( FBJ murine osteosarcoma viral (v-fos) oncogene homolog, FOS) [FOS] Alleles: A1, A2, B2, B1 |
| Insert Size (kb) |
1.300 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests analyzed on agarose gels give the following sizes (kb): PstI--4.4, 1.0, 0.2; BamHI--5.7; EcoRI--5.7; HindIII--5.7. The 1.0 kb PstI fragment is usually used for the fos probe. BglII/PvuII fragment of pFBJ-2 dC tailed into dG tailed pBR322 cut with PstI; contains internal PstI (2), StuI NcoI, SstI, SalI sites; PstI-cut plasmid yields 1.0, 0.26, and 0.06 kb fos-specific fragments. |
| References |
Curran T, et al. FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active provial DNA. J. Virol. 44: 674-682, 1982. PubMed: 6292525
Tom Curran, personal communication
|
