| 產品名稱 |
pHIIF-SV-gamma1 [HIIF-A] |
| 商品貨號 |
B180926 |
| Designations |
pHIIF-SV-gamma1 [HIIF-A] |
| Species |
Homo sapiens, human |
| Depositors |
Biogen N.V., Biogen, Inc., Biogen N.V. |
| Applications |
produces protein interferon gamma |
| Vector |
Construct size (kb): 9.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSV529
Intact vector size: 8.640
Type of vector: plasmid
Vector end: BamHI (C-tailed)
Vector end: BamHI (C-tailed)
Cloning sites: BamHI EcoRI
Polylinker sites:
Construction: pBR322, SV40, pSV52
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40 late
replicon: pMB1, SV40
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNG
Genomic copy number: unique
Gene name: interferon, gamma
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 q24.1
Tissue: splenocytes, mitogen-stimulated
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 1.1
Cross references: Insert lengths(kb): 1.100000023841858 Tissue: splenocytes, mitogen-stimulated Gene product: interferon gamma [IFNG] Target Gene: interferon gamma |
| Insert Size (kb) |
1.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--9.2, 5.5, 2.7, 1.1; BglI--3.3, 2.5, 1.35; NcoI--4.4, 3.1, 1.9. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.86; NcoI--0.9. Analysis of the undigested plasmid shows a significant band migrating at approximately 2.7 kb and a weaker band at 5.5 kb. Contains the complete coding sequence, between nucleotides (approx) 100 and 600, with expression regulated by the late SV40 promoter in transfected cells. |
| References |
Fiers WC, Allet B. DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields. US Patent 5,004,689 dated Apr 2 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
