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<em>Chlamydia trachomatis</em>

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價格:詢價

編號:B180947

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

Chlamydia trachomatis

商品貨號

B180947

Classification

Chlamydiaceae, Chlamydia

Deposited As

trachoma serotype k

培養基

Agent

Chlamydia trachomatis

Strain

Trachoma type K strain UW-31/Cx

Applications

Note that activities with high potential for aerosol production require BSL 3 facilities and practices.

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen

Storage Conditions

-70°C or colder

Comments

Closely related immunologically with LGV Type L III.

Note that activities with high potential for aerosol production require BSL 3 facilities and practices.

Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour.

Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 ug/mL cycloheximide. Incubate at 37°C for 48 hours.

Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC^® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.

Effect on Host

Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa

Recommended Host

McCoy cells (ATCC^® CRL-1696™)

HeLa 229 cells (ATCC^® CCL-2.1™)

Growth Conditions

Temperature: 36.5°C

Incubation: 2 days at 37°C

Name of Depositor

SP Wang

Source

Human cervix, cervicitis, Seattle, WA