Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Closely related immunologically with LGV Type L III.
Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour.
Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 ug/mL cycloheximide. Incubate at 37°C for 48 hours.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Recommended Host
McCoy cells (ATCC® CRL-1696™)
HeLa 229 cells (ATCC® CCL-2.1™)
Growth Conditions
Temperature: 36.5°C
Incubation: 2 days at 37°C
Name of Depositor
SP Wang
Source
Human cervix, cervicitis, Seattle, WA
References
Kuo CC, et al. TRIC type K, a new immunologic type of Chlamydia trachomatis. J. Immunol. 113: 591-596, 1974. PubMed: 4210884
