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pCMV HSV S2P plasmid in <em>Escherichia coli</em>
pCMV HSV S2P plasmid in <em>Escherichia coli</em>
規格:
價格:詢價
編號:B181046
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCMV HSV S2P plasmid in Escherichia coli
商品貨號
B181046
Designations
pCMV HSV S2P plasmid in Escherichia coli
GenBank Number

AF019612

培養基
Species
Homo sapiens, human
Depositors
JL Goldstein
Applications
encodes an epitope tag for protein isolation or monitoring S2P
Vector
Vector: pcDNA3
Vector type: phagemid
Vector size (kb): 5.4
Markers: ampR
Features: CMV promoter, T7 and SP6 promoters, SV40 replicon, G418 resistance, pMB1 replicon
Insert
Insert size (kb): 1.7
Gene: S2P
DNA: cDNA
Source : human
Genbank accession: AF019612
Insert end 5’: NheI
Insert end 3’: XbaI
Insert Size (kb)
1.7
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Freeze dried E. coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): NheI/XbaI--5.4, 1.7; PstI--4.0, 3.1; ClaI--7.1; EcoRI--7.1.
The insert contains the following restriction sites (approximate kb from the 5' end): StyI--0.36; DraII--0.90; BglI--1.40; SacI--1.46.
The insert contains human S2P with HSV epitope tag at the amino terminus.
References

Rawson RB, et al. Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs. Mol. Cell 1: 47-57, 1997. PubMed: 9659902

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