Insert size (kb): 1.7 Gene: S2P DNA: cDNA Source : human Genbank accession: AF019612 Insert end 5’: NheI Insert end 3’: XbaI
Insert Size (kb)
1.7
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Freeze dried E. coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): NheI/XbaI--5.4, 1.7; PstI--4.0, 3.1; ClaI--7.1; EcoRI--7.1.
The insert contains the following restriction sites (approximate kb from the 5' end): StyI--0.36; DraII--0.90; BglI--1.40; SacI--1.46.
The insert contains human S2P with HSV epitope tag at the amino terminus.
References
Rawson RB, et al. Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs. Mol. Cell 1: 47-57, 1997. PubMed: 9659902
